[et_pb_section fb_built=”1″ _builder_version=”4.14.2″ background_image=”https:\/\/lma.unizar.es\/wp-content\/uploads\/2021\/06\/fondo2.jpg” custom_padding=”260px||85px||false|false” global_colors_info=”{}”][et_pb_row _builder_version=”4.3.2″ global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.3.2″ global_colors_info=”{}”][et_pb_text _builder_version=”4.9.4″ header_font=”|||on|||||” header_text_align=”center” header_text_color=”#ffffff” header_font_size=”50px” header_text_shadow_style=”preset4″ global_colors_info=”{}”]<\/p>\n
[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ _builder_version=”4.14.2″ _module_preset=”default” background_color=”#f2f2f2″ custom_margin=”-1px|||||” custom_padding=”10px||30px|||” box_shadow_style=”preset3″ locked=”off” global_colors_info=”{}”][et_pb_row _builder_version=”4.14.2″ _module_preset=”default” background_color=”#FFFFFF” custom_margin=”12px|auto||auto||” border_width_all=”10px” border_color_all=”#4370b4″ border_style_all=”outset” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.14.2″ _module_preset=”default” global_colors_info=”{}”][et_pb_text _builder_version=”4.14.2″ _module_preset=”default” text_font=”|700||on|||||” text_font_size=”18px” text_orientation=”center” custom_margin=”||13px|||” global_colors_info=”{}”]Scanning probe microscopy (SPM) area[\/et_pb_text][et_pb_button button_url=”https:\/\/lma.unizar.es\/en\/scanning-probe-microscopy-spm-area\/” button_text=”SEE EQUIPMENT” button_alignment=”center” _builder_version=”4.14.2″ _module_preset=”default” custom_button=”on” button_text_size=”16px” button_bg_color=”#FFFFFF” button_border_width=”0px” button_border_radius=”4px” button_letter_spacing=”0px” button_font=”|700||on|||||” button_icon=”=||divi||400″ custom_margin=”0px||8px||false|false” custom_padding=”4px||4px||true|false” hover_enabled=”0″ button_text_color_tablet=”” button_text_color_phone=”” button_text_color_last_edited=”on|desktop” global_colors_info=”{}” button_bg_color__hover=”#4370b4″ button_bg_color__hover_enabled=”on|hover” button_text_color__hover=”#FFFFFF” button_text_color__hover_enabled=”on|hover” button_border_color__hover=”#4370b4″ button_border_color__hover_enabled=”on|desktop” sticky_enabled=”0″][\/et_pb_button][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ _builder_version=”4.3.2″ custom_padding=”23px||55px|||” global_colors_info=”{}”][et_pb_row _builder_version=”4.9.4″ max_width=”75%” custom_padding=”||21px|||” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.3.2″ global_colors_info=”{}”][et_pb_text _builder_version=”4.9.4″ _module_preset=”default” global_colors_info=”{}”]<\/p>\n
AFM is probably the less invasive probe in the toolbox of Nanotechnology. In particular, it is able to profile a sample with great accuracy and applying small enough forces as to not damage biological materials. Characterizing the behaviours of single biomolecules by AFM provides considerable novel insights into the underlying mechanisms guiding life activities, contributing much to cell and molecular biology.<\/p>\n
The possibility to scan in aqueous media and to perform force spectroscopy, allows us to address the analysis of enzymatic mechanisms, molecular recognition pathways, regulatory processes and forces that govern biosystems using AFM in buffer at the single-molecule level. We often make use of the identification of receptors on substrates and on cellular and bacteria membranes using functionalized tips with proper receptor-binding ligands.<\/p>\n
[\/et_pb_text][et_pb_image src=”https:\/\/lma.unizar.es\/wp-content\/uploads\/2021\/11\/AFM-materiales-biologicos-1.jpg” title_text=”AFM-materiales-biologicos-1″ align=”center” _builder_version=”4.9.4″ _module_preset=”default” custom_margin=”||26px|||” border_width_all=”1px” global_colors_info=”{}”][\/et_pb_image][et_pb_text _builder_version=”4.14.2″ _module_preset=”default” text_font=”|700|on||||||” text_font_size=”14px” global_colors_info=”{}”]<\/p>\n
AFM images of single human GalNAc-T3 molecules showing different conformations upon ligand binding and catalysis at near physiological conditions to unveil the catalytic mechanism of this two-domain enzyme.<\/p>\n
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Another primary line of research pursues the study of model cell membranes incorporating phospholipids, sterols, glycosphingolipids, proteins, etc… under controlled environments available at the AFM facility. These membranes are prepared using the Langmuir-Blodgett and Langmuir-Schaefer technologies. We can then study their morphology and nanomechanical properties in air or liquid media. La interacci\u00f3n de estos modelos de membranas celulares con nanopart\u00edculas, f\u00e1rmacos o xenobi\u00f3ticos es tambi\u00e9n un campo de inter\u00e9s en los laboratorios LMA.<\/p>\n
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The application of an alternating magnetic field to a model cell membrane on which magnetic nanoparticles have been located, induces a local heating that allows a fluidization of the membrane and penetration of the nanoparticles through the membrane.<\/p>\n
[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ _builder_version=”4.9.4″ _module_preset=”default” min_height=”179px” custom_margin=”9px||-9px|||” custom_padding=”0px||4px|||” saved_tabs=”all” global_colors_info=”{}”][et_pb_row use_custom_gutter=”on” gutter_width=”4″ _builder_version=”4.9.4″ _module_preset=”default” max_width=”75%” custom_margin=”0px|auto||auto||” custom_padding=”55px||||false|false” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.9.4″ _module_preset=”default” global_colors_info=”{}”][et_pb_gallery gallery_ids=”2911,2910,3029″ posts_number=”3″ show_title_and_caption=”off” show_pagination=”off” module_class=”ilogos” _builder_version=”4.9.4″ _module_preset=”default” global_colors_info=”{}”][\/et_pb_gallery][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ _builder_version=”4.9.4″ _module_preset=”default” use_background_color_gradient=”on” background_color_gradient_start=”#000000″ background_color_gradient_end=”rgba(0,0,0,0.48)” background_color_gradient_overlays_image=”on” background_image=”https:\/\/lma.unizar.es\/wp-content\/uploads\/2021\/10\/render-lma-1.jpg” custom_margin=”9px|||||” custom_padding=”||42px|||” saved_tabs=”all” global_colors_info=”{}”][et_pb_row column_structure=”1_3,1_3,1_3″ use_custom_gutter=”on” gutter_width=”2″ _builder_version=”4.9.4″ _module_preset=”default” max_width=”75%” global_colors_info=”{}”][et_pb_column type=”1_3″ _builder_version=”4.9.4″ _module_preset=”default” global_colors_info=”{}”][et_pb_text _builder_version=”4.9.4″ _module_preset=”default” text_text_color=”#FFFFFF” text_line_height=”2em” global_colors_info=”{}”]<\/p>\n
Laboratorio de Microscop\u00edas Avanzadas\n<\/p>\n
We are a unique initiative at national and international levels. We provide the scientific and industrial community with the most advanced infrastructures in Nanofabrication, Local Probe and Electron Microscopies for the observation, characterization, nanopatterning and handling of materials at atomic and molecular scale.[\/et_pb_text][\/et_pb_column][et_pb_column type=”1_3″ _builder_version=”4.9.4″ _module_preset=”default” global_colors_info=”{}”][et_pb_text _builder_version=”4.9.4″ _module_preset=”default” text_text_color=”#FFFFFF” link_text_color=”#FFFFFF” global_colors_info=”{}”]<\/p>\n
Contact information<\/p>\n
Campus R\u00edo Ebro, Edificio Edificio I+D+i<\/p>\n
Tel.:(+34) 976 762 980 <\/strong><\/a><\/span>
lma@unizar.es<\/strong><\/a><\/span>