LMA

Nanobiology and new nanobiomaterials

  Nanobiology and new nanobiomaterials

 

In this research area, we work in this three fields:
  • Molecular recognition. The goal is the measurement of the intermolecular forces that govern the recognition processes between biomolecules using force spectroscopy. By careful analysis of the breaking force at various pulling speeds, it is possible to map the energy landscape of the chemical bond under mechanical force. This leads to interesting results in the study of antibody-antigen, protein-protein, protein-DNA and even protein-living cell interactions at the single-molecule level. We are also working in the analysis of different biological processes under relevant physiological conditions using AFM imaging to know the models of binding and estimate parameters on the formation of the complexes.
Molecular Recognition
Force spectroscopy of the detachment of a protein-ligand bond.
  • Ferritin-based nanomaterials. Preparation of nanoparticles in the cavity of the protein ferritin. These nanomaterials are used as a model for studying the physical properties at very low temperatures of particles with a size intermediate between the atom and the macroscopic magnets. These NPs can be applied to the area of biomedicine.
Ferritin
AFM images of individual Ferritin proteins and their assembly in artifical structures using a Dip Pen approach.
  
  • Dip-pen nanolithography (DPN). This is a scanning probe lithography technique where an AFM tip is used to directly transfer reagents to a surface via a solvent meniscus. DPN is used to organize a great variety of molecules and particles on different surfaces with nanoscopic resolution. We are positioning ferritin containing nanoparticles in the most sensitive areas of new microsensors to explore the signal of a few NPs, and nanostructuring these materials for the study of their properties on surface.