Scanning Probe Microscopies are key enabling techniques in Nanoscience and Nanotechnology, supporting a wide range of multidisciplinary activities. The LMA hosts a multipurpose AFM/STM head mounted on a vibration isolation damping table which covers a broad range of applications at near ambient conditions.

On top of high-quality topographic imaging in contact and dynamic modes, the Multimode 8 from Veeco-Bruker is a Scanning Probe Microscope equipped with KPM (Kelvin Probe Microscopy), c-AFM (conductive-AFM), PFM (Piezo-Response Force Microscopy) liquid and electrochemistry cells, PicoForce module for force spectroscopy measurements, variable Peltier temperature controller (-22 ºC to 200 ºC), Torsion mode head and QNM (Quantitative Nanomechanical Property Mapping) with available Peak Force mode.

The following information can be obtained with our SPM instruments:

  • Surface morphology. Topography with resolution below 1 nm.
  • Electrical conductivity (c‐AFM). Quantitative local electrical resistance measurements.
  • Mechanical properties (QNM Peakforce). Quantitative local measurement of the elastic modulus, adhesion, stiffness,
  • energy dissipation and deformation on surfaces.
  • Local electrical potential (KPM). Qualitative measurements of local charge distribution.
  • Magnetic properties (MFM). Magnetic properties analysis under magnetic fields.
  • Closed-loop long-range Z-scanner (Pico Force) for force spectroscopy on large biomolecules
  • Nanomechanical studies by using Single-Molecule Force Spectroscopy. Pull‐push experiments for inter and intra-
  • molecular force measurements, with 1 pN resolution.
  • Electro-chemical properties (EC‐SPM). Study of chemical reactions on surfaces under controlled environments.
  • Piezo-electric properties (PFM). Using the tip as electrode and deformation sensor.
  • Thermal dependence. Capacity to measure in the 250 K to 500 K range.
  • Topography based in ambient Scanning Tunnel Microscopy (STM).

The sample should be immobilized onto a flat substrate (for instance, biomolecules should be immobilized onto a mica surface via adsorption or via a covalent procedure).
The sample should exhibit a lower roughness than the range of the piezo scanner.
The size of the sample should be small enough to fit inside of the microscope, around 1 cm2 in surface and 0.5 cm in thickness.

Types of samples that can be studied with the environmental SPMs include:

  • Biological samples (DNA, proteins and peptides; cells, viruses and bacteria; biological tissues, etc.).
  • Organic and inorganic thin films.
  • Gels and Polymers.

Environment: Air, liquid and electrochemical cell
Temperature range: [-35, 200] ˚C
Rango escáner: J) 200 µm x 200 µm x 5 µm E) 12 µm x 12 µm x 3 µm
Magnetic field: No

Fig 1: Air topography of Rizobacteria

Fig 2: Graphene nanoflakes on mica

Fig 3: Topography and magnetic signal (phase contrast) from a focus ion beam fabricated nanostructure.
Fig 4: Simultaneous Topography and electrical conductivity from nanoelectrodes.
Fig 5: Force spectroscopy of proteins with a biofunctionalized AFM tip

Laboratorio de Microscopías Avanzadas

We are a unique initiative at national and international levels. We provide the scientific and industrial community with the most advanced infrastructures in Nanofabrication, Local Probe and Electron Microscopies for the observation, characterization, nanopatterning and handling of materials at atomic and molecular scale.

Contact information

Campus Río Ebro, Edificio Edificio I+D+i

C/ Mariano Esquillor, s/n
50018 Zaragoza (España)

Tel.:(+34) 976 762 980